I am developing liposomes entrapping a hydrophobic drug. To check whether the liposomes itself are toxic or not, what concentrations of it must be tested on the cells?
Moreover, is there any maximum acceptable limit of cell death (for e.g. 5% or 10%) up to which we can say that the liposomes are not toxic?
Compare the IC50 (inhibitory concentration needed for 50% cell kill) for loaded vs. unloaded liposomes. Be sure to use the same cell viability assay to compare both. All drugs are toxic to some extent, what is really important is the potency - what is the minimal amount of drug to get the maximal response. If your empty liposomes kill 50% of the cells but your loaded particles kill 99.9% - that MAY be an acceptable kill depending upon the applications. Ideally, you like that ratio to me much different (less kill with unloaded) but compare many anti-cancer drug toxicity profiles and you will see that few drugs are "non-toxic" and exclusively specific. As you move forward, biologically (in-vivo) determining a MTD (maximum tolerated dose) and comparing it to the potency allows one to calculate the therapeutic index of the material. That's what is critical.
so you mean That I have to prepare the free liposome in same concentration as the loaded drug and compare the cell viability on cells for both of them?
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