Tips for qPCR primer designing for "small" and "full of variant" genes?

10 April 2020 0 6K Report

Hi! I have a problem during designing primers for qPCR for genes such as:

GSTA1

GSTP1

TXN (TRX1)

The problem is that they have many snips on their exonic regions (based on ensemble) and also, they are small and highly similar to some other genes. So, I have fewer options and the primers designed using primer BLAST have many off-targets that are expressed in the tissues that I am studying. Any recommendations?

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