Time and Tº on tissue samples using Sigma LPL activity kit?

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Does variations in the number of lattice nodes affect lift and drag calculation in Lattice Boltzmann Method?

Dear colleagues, I have spent a few weeks trying to figure this problem out with no success, so that I post this question here. First of all, I have to say I am new to LBM method. I am working...

11 June 2024 1,084 3 View

Can a pegmatitic gypsum holds Lithium content ?

Hi to all, Just a question, knowing the brines origin of the gypsum and having found pegmatitic clusters of gypsum, with crystals up to 10-12cm, and knowing the ionic interactions of the elements...

13 May 2024 3,657 0 View

What unit is U/s in anton paar rheometer?

Hello everyone, I'm trying to obtain curves of friction coefficient as a function of entrainment speed using a tribology setup geometry in an Anton Paar rheometer. However, the software gives me...

01 May 2024 4,089 2 View

What is Crisis Intervention Management?

I need to know what will I present on this topic

18 April 2024 563 1 View

Marine environmental layer datasets?

Hello everyone! I've been having some difficulty in finding datasets for specific variables: - Dissolved oxygen - Upwelling index - Sea surface Salinity I was hoping of getting datasets of...

15 April 2024 5,354 2 View

For free energy of solvation using GROMACS, in a molecule with charge -6 and 100 atoms, ¿how many lambdas are necessary to obtain a good result?

In the calculation of free energy of solvation using GROMACS, for a molecule with negative charge (-6) and approximately 100 atoms, ¿how many lambdas are necessary to calculate the free energy...

03 April 2024 316 0 View

Interested in river ecology? looking for a PhD grant or postdoctoral position?

Send me an e-mail for more details at [email protected]

15 March 2024 3,940 0 View

When does a research becomes mixed methods?

I am considering undertaking a quant + qual research by providing teachers with a questionnaire to measure their feelings and practice with regards to a type of music. I was thinking of...

14 March 2024 5,757 11 View

How to properly remove tegaderm in a wound healing mice model?

Dear scientists, we are currently carrying out a wound healing mouse model. The process is as follows: make the wound, apply and suture the silicone splint, put the tegaderm and apply topically...

06 March 2024 6,576 1 View

Do you have any suggestion of some topic thesis related to chemistry?

If you have some ideas kindly list down here. Thank you

30 January 2024 8,692 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Alaa Jabbar Abd Al-Manhel

Dear sir , see this link its useful

http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/mak109bul.pdf

http://www.sigmaaldrich.com/technical-documents/articles/biology/roar-assay-kits.html

Francisco Algaba-Chueca

Thank you so much dear friend. Im going to read it and I hope it will be useful.