Hello,
Does anyone have any thoughts or experience regarding different light cycles for plant tissue culture? It seem that 16hr light/8hr dark is the standard for tissue culture, but I haven't come across much literature discussing why this is the case. My current focus is maximizing multiplication rates, and I am considering a trial with more light time and less dark time. Does anyone have any insight on positive or negative effects of altering the light cycle, or any good sources that discuss this aspect of the in vitro environment?
Dear Kasey Greenwald,
I have experience in Banana and pineapple tissue culture. For both I am providing 8 hr light/ 16 hr Dark. It can help to reduce the cost. But I have not studied the multiplication rates for both crops. It will be a interesting topic to study, but this is having economical aspect to reduce cost for electricity.
S. V. G. Nirosha Priyadarshani, I didn't understand your answer, 8 hr light / 8 hr darkness makes 16 hr in total.
Sorry It was a mistake. I corrected it to 8 hr light/16 hr Dark
Anyway Buhara, 8/8h photoperiod have been also used. I can't remember the reference: I'll check in my records.
Hello Dear,
If you change the light duration from standard light duration, it will definitely effect on morphology and flowering and reproduction of plants. Some previous literature are already there regarding this light effect on tissue culture.
You need to tell which plant you are working with. When hardening is required before rooting, higher light extensity is required.
Butall depends on the plant chosen.
With some plants you also need more light intensity while they come from tissue culture state( small plantlets) to shoots which should develop stronger leaves. There we use 3x the amount of light
My experience is light cycle/ intensities does not have much impact on multiplication rate for woody plant. Change in hormone or basalt salts or even organic compounds make the different. Light would mostly change the elongation and leaves quality; would cause or eliminate vitrification for some species.
S. Wenkart : Your suggestions are very interesting. I have seen research increased light intensities upto 150 PPFD during root initiation phase and reduce to 50 PPFD when start acclimatization/ Hardening off.
I am not fully understand "need more light intensity while they come from tissue culture state( small plantlets) to shoots which should develop stronger leaves. ": were you mean during shoot development in TC state (inside the vessel) or the transition between in vitro to ex- vitro?
I meant: the small shoots need to get stronger and maybe develop bigger leaves. With Jojoba we achieved this on medium with chacoal and more light. But every plant is different...
- Badges
- Science topic