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PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

How many indicators do I need to represent a latent construct?

Hi folks! We did a large survey and planned to use 3 indicators to represent a latent construct (Sense of Place/SoP) in a latent regression model (structural equation modeling). However, one of...

15 February 2021 7,705 3 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

What is the structure of a photon that can describe the interference experiments that reject the wave nature of light?

De Broglie's double-solution postulated a causal model of Quantum Mechanics. Knowledge of the photon structure was needed to model the particle part of the cause of the Double Slit interference...

11 February 2021 6,869 5 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

What is the link between anthropology and ethnobotany?

27 December 2020 282 3 View

How ethnobotany is linked to climate change?

25 December 2020 8,180 3 View

What is the role of Ethnobotany in food security?

25 December 2020 6,086 3 View

Did old ethnobotany really mention anti-tumor herbs? Was the cancer/ tumor identified in olden times??

09 June 2020 7,920 4 View

What are the statistical parameters required for quantitative Ethnobotany?

I have started a survey cum field study on the use of plants by local people of a particular locality. I have also documented the Phytodiversity of that location. Please suggest some statistical...

10 January 2020 8,626 12 View

What are the statistical parameters required for quantitative Ethnobotany?

I have started a survey cum field study on the use of plants by local people of a particular locality. I have also documented the Phytodiversity of that location. Please suggest some statistical...

31 December 2019 3,510 6 View

What statistical formulas can be used in Quantitative ethnobotany?

what statistical formulas can be used in Quantitative ethnobotany?

29 June 2019 8,222 6 View

What statistical formulas can be used in Quantitative ethnobotany?

what statistical formulas can be used in Quantitative ethnobotany?

05 June 2019 5,606 4 View

Can you suggest me a good Publisher in India dealing with Ethnobotanical books?

I am at present finalizing my manuscript on the Ethnobotany of a paricular area in India based on my own research. Please suggest me a good publisher in this regard who can publish my book.

03 April 2019 501 8 View

Can anybody provide the full paper reffered as "Khan Arthur Ali, 2005. Ethnobotany in twenty first century in India, Ethnobotany, 17:71-78." ?

It's a much needed paper

11 December 2018 8,143 0 View

Evans Ehouman

There is an interesting paper published at Article Anti-Trypanosoma cruzi activity of 10 medicinal plants used ...

It may be a good source of information.

Kind regards,

Evans, E.

Arvind Singh

Please take a look at the following RG links.

Article Asteraceae Plants as Sources of Compounds Against Leishmania...

Article Potential therapeutic use of herbal extracts in trypanosomiasis

Article Natural products and Chagas' disease: The action of triterpe...

Article Antioxidant effect of Morus nigra on Chagas disease progression

Chapter Can the Cure for Chagas’ Disease be Found in Nature?