There is two users or programs who try to access the cache memory. How does Xen scheduler differentiate between the probe and normal action? I mean how does xen know that user 1 which try to access cache memory launch a probe attack or not?
i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...
07 July 2024 2,946 1 View
Hello researchers, i need some help, i am packaging Lentivirus( having my transgene) in HEK293T cells, as I will collect the supernatant and concentrate my lentivirus,I also want to check...
15 June 2024 1,596 3 View
i need some help setting up my culture plate having negative controls,I am going to infect HEK293T cells in a 6-well plate... to one well I will add my lentivirus particles(packaged already), and...
15 April 2024 7,218 5 View
Hello dear researchers,i have transduced my HEK293T cells with IL-2 and then puromycin screening was done, the cells were showing good egfp flourescence ,but then due to some reasons I have to...
14 April 2024 7,285 2 View
I received comments from a reviewer on my manuscript having RCT design and I compared pre and post data by pair t- test. The reviewer commented: Effect size indices and 95%CI could be...
26 January 2024 1,980 2 View
Hello to all of the researchers, i need to isolate and grow T lymphocytes from swine lymph nodes. I need you people to guide/help me or send me any protocol/video for T cells isolation from wine...
24 January 2024 1,380 1 View
Hello dear researchers, i am generating a stable cell line of HEK293T cells using lentivirus, i want to ask about the puromycin kill curve,if i seed my cells in a 12-well plate on day 1, Q1) when...
04 January 2024 3,109 4 View
Hello, i have grown my HEK293T cells in 4 wells of a 24-well plate and added different serial dilutions of lentivirus(containing my gene and GFP), now i want to perform FACS analysis so for this...
30 November 2023 8,866 2 View
Hello to all researchers, i need to do lentivirus titration and for this I need 10 fold serial dilution i.e 10^-1 to 10^-4...and i have my lentivirus stock stored with me...second thing is if i...
28 November 2023 9,880 1 View
i have my gene of interest in this way(picture in attached files), Now I am confuse about whether i can use this whole thing to construct my one the 3 lentivirus packaging vector named pLenti...
03 September 2023 7,906 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
all math can be traversed by code? all math can be translate to code?
26 July 2024 9,530 0 View
I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...
22 July 2024 3,794 1 View
Hey all, I need help testing for multivariate outliers using STATA for my master thesis. The literature recommends the Minimum Covariance Determinant (MCD) (Verardi & Dehon, 2010). I found the...
22 July 2024 8,821 2 View
Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...
21 July 2024 1,632 0 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
Hi all, Do you know how to plot this figure? I found many of them in Nature journals but have not figured out how to make them. Which software/tool do you suggest? Thank you very much.
15 July 2024 8,384 2 View
How can novel network architectures, such as software-defined networking (SDN) and network function virtualization (NFV), contribute to improved handover performance and reduced latency in future...
12 July 2024 6,183 1 View