Dear Mohammad Mirdoraghi,

The first question would be: How did you verify these are PCE's and NCE's and not something that would me multilobular like granulocytes? Further: How many minutes between aspiration and fixation? If >5 minutes, this would induce a risk of degeneration of the cells. Also, the concentration of formalin/other fixation liquid is important as well as the manual technique in making the slide. And last: In a cytology slide like this, a less-than perfect state of the cells is quite common and is not necessarily caused by an error in method. I would recommend thinprep or cellblock (paraffin-embedded cytology) to increase quality.

Regards