What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

18 October 2020 6,639 3 View

Sampling rate in matlab?

Can I know what is the sampling rate of ifft() command which is a build in function in matlab?

01 June 2020 7,937 12 View

Finding Hidden Defect in ceramic sample in matlab?

I am dealing with ' Optimal signal processing of frequency-stepped CW radar data' from the website of Optimal Signal Processing of Frequency-Stepped CW Radar Data I want to find the hidden...

27 May 2020 1,125 12 View

Any experts in NVIVO 12 Plus for Qualitative data analysis?

I have interview contents that need NVIVO to compile and provide tools for data analysis and presentation. Need experts to share software application experience, pros and cons, advantages and...

01 March 2019 8,723 4 View

Which journals in applied linguistics have short turnaround times? How long was your longest wait...?

Which journals in applied linguistics have short turnaround times? How long was your longest wait from the first draft submission till the first substantial response from the editor / reviewers?

16 December 2018 5,102 12 View

Who knows this green flower?

05 November 2016 2,899 20 View

What is the effect of N-glycosylation on protein mobility in western blot?

02 November 2016 9,727 2 View

Which lysis buffer should I use for blotting proteins found in the ER and Golgi?

I am looking to see if various proteins of interest are mis-glycosylated, and trapped in the ER or Golgi apparatus. Initially, my experiments have been focussed on performing western blots to look...

29 October 2014 8,893 3 View

What is a potential sequencing technology to read through heterochromatic regions, where long repetitive sequences reside?

See a Research Topic, entitled "Marching toward 100% whole genome sequencing", in the link below. I would be happy to receive and to share relevant articles.

05 June 2014 159 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View