The rejection of ion at higher feed solution pH in FO is likely due to solely size exclusion or electrostatic repulsion or both?

More Jeng Yih Law's questions See All

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View

Can you explain me the Leadership Styles of Jesus as Found in the Four Gospels?

I want to get more information about the Leadership Styles of Jesus as Found in the Four Gospels

02 December 2023 3,729 3 View

Are there other methods that can be used to identify and quantify hemolysis, icterus, and lipemia (HIL) interferences?

Farell & Carter (2019) state that visual inspection for changes in serum or plasma color and clarity has traditionally been used to determine and quantify HIL interference. Any turbidity,...

22 February 2023 2,430 1 View

Microwave applicator instruction manuals needed?

I am working energy calculations for microwave-assisted synthesis of Au and Ag NP. I am looking for instruction manuals for the following microwave applicators used in microwave-assisted synthesis...

11 February 2023 4,644 0 View

Need journal recommendations?

Hi academics, I am looking for a journal that accepts papers on qualitative textual and multimodal discourse analysis of digital game dialogues. Discussion is related to social (and eco-)justice....

05 February 2023 9,588 4 View

Can I use a low-cost microscope to identify microbes in soil that make polyhydroxyalkanoate (PHA)?

I am interested in doing experiments at home related to PHA production. First, I want to try if I may use a low-cost microscope alongside using the serial dilution technique to find out whether a...

26 July 2022 2,795 4 View

How to calculate Buy and Hold Abnorml Return?

Hi everyone, I am doing my research related to IPOs long term performance. For the BHAR formula, I just want to confirm the formula is that always compared with the first trading day price, or...

21 October 2021 7,054 2 View

How to perform a low volume protein precipitation?

I have a start volume of 530ul crosslinked protein sample. I want to precipitate it. The Methanol/Chloroform and acetone methods require quite a bit of volume for precipitation, making it so that...

14 March 2021 7,824 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 6,216 3 View

Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

18 October 2020 6,885 3 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View