hellow everyone, nowadays i spend a much time thinking p22 phage in relation to transduction.
these days,i have heard that the p22 phage which i used have an only lytic cycle pathway. i wonder about the reason why i use p22 phage which lives on only lytic cycle at the time i experiment with transduction.
i guess i should better use a lysogenic life cycle pathway in order to insert a gene of interest, if i experiment a transduction as you know.
is there any other meaning of using an only lytic cycle p22 phage for recombination? i guess that the experiment which i implemented was not to insert a gene into salmonella chromosom, then i think this is not to do that....
please help me and teach me the reason.
thank you for reading my question and have a nice day.
I'm not sure I'm understanding your question completely, but I think what you're asking is why you would use a lytic mutant of P22 for transduction instead of a lysogenic one.
As I understand it, typically the point of transduction is to conveniently transfer large stretches of DNA from strain A to strain B, not to infect strain B with a prophage. If you use a phage that's always lytic, then it will simply kill off cells infected with the actual phage genome. Cells that have been infected with a phage that accidentally packed some of its previous host's genome into the capsid will survive because they haven't actually been injected with the phage genome.
That way, you can be pretty certain that any surviving colonies probably have taken up some DNA from the previous host and may have recombined it into their own genome.
If you tried to use a lysogenic phage for this, you would probably end up with a lawn of surviving cells, very few of which will actually have acquired your DNA of interest. The vast majority will be phage lysogens.
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