My extraction buffer (sodium phosphate buffer) contains 0.3 mmol l-1 dithiothreitol (DTT), 0.2 mmol l-1 ethylenediaminetetraacetic acid (EDTA), 10 mmol l-1 sodium metabisulphite and 0.2% (w/v) Triton X-100. (Based on previous literature- for the same sample that I'm gonna analyse)
In subsequent analysis, I have to do Bradford assay and most of the readily available Bradford assay reagents have compatibility concentrations. (E.g. upto 0.1% - Triton X-100 if we use Bio-Rad Bradford reagent)
My question is can I reduce the initial concentration of Triton X-100 to less than 0.1% (around 0.05%)? Will it affect the enzyme extraction process?
How should this problem be approached?
Perhaps you can dilute the sample with water to reduce the Triton X-100 concentration prior to using it in the protein assay.
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