The protocol to assay mRNA stability from E.coli?

More Garam Wi's questions See All

Help with using chi squared to assess how the rate of missing data differs between the control and intervention group in a study?

Good evening, newbie to Statistics here I currently have a 2x2 table showing the number of participants who did and did not provide data from two groups (control and intervention). Is the...

20 November 2022 7,830 7 View

What causes wrinkles in thin films to disappear after heat treatment?

When I anneal the free standing thin film (>300 degree Celsius), wrinkles of the thin film disappear. What is the principle of this phenomenon? (This thin film is can be a single layer or a...

28 December 2019 8,571 4 View

What happens to the mechanical properties when annealing a metal thin film?

If the metal thin film is annealed, How do the mechanical properties such as tensile strength, hardness, Young's modulus change? The metal thin film is not alloy and thickness is less than 100...

21 August 2019 8,227 3 View

Cloning : Enzyme site is always cut by something ?

Hello everyone, thesedays i have serious problem in cloning. I usually do T-vector cloning with insert before I ligase into expression vector.T-vector cloning is comfirmed by sequencing but the...

14 November 2016 6,450 4 View

How can I get Seebeck coefficient by doping with BoltzTraP?

Hi,all. I am a student in master course starting to learn about thermoelectric materials. Due to advice on this website, I can get thermoelectric properties with BoltzTraP and...

16 March 2016 6,757 14 View

Could I know and adjust the transport direction from VASP?

Dear, Hello. I am a junior studying DFT for thermoelectric materials. I am in the elementary step for DFT calculation. I can only get VASP outputs for some materials. The question is "how can I...

21 January 2016 7,456 2 View

How could I make BoltzTrap input files from VASP outputs?

Hello. I am a beginner in this field. I want to calculate thermoelectric properties with BoltzTraP. I've finished setup of BoltzTraP and ASE and I've run examples. But I have a problem with...

01 November 2015 8,505 17 View

How could I make super cell and transform the coordinates of unit cell using VESTA?

Hello. Superiors. I want to make super cell structure to calculate band structure. I have been searching for it and found the paper attached (E.S.Tasci, "How to prepare an input file for surface...

30 September 2015 1,526 31 View

What is the common instrument to detect IV curve?

Hi there, I am planning to having an instrument for IV curve measurement. Could you please suggest to me what is the instrument model which commonly and easily be used for IV curve detector for DSSC.

01 January 1970 8,441 6 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Anna Konovalova

You can follow the mRNA stability by doing by a Northen blot or qPCR (less ideal) after transcription (new mRNA synthesis) is inhibited (with rifampicin).

Igor Oscorbin

Here you can find information about RNA quality assesment before qPCR.

https://www.nature.com/nprot/journal/v1/n3/full/nprot.2006.236.html

Here valuable recomendations about qPCR in general and work with RNA as starting material for qPCR experiments.

http://clinchem.aaccjnls.org/content/55/4/611.long