These images were the immunofluorescence of my protein in the hippocampus. The images represent the merged signals with DAPI signals (blue),my protein(red), astrocyte marker glial fibrillary acidic protein (GFAP,green).thanks.
Víctor Calbiague Garcia
Hi,
Although -in my opinion- the IF are some subjective experiments, I can see a notorious colocalization of green and red markers in some cells, but I am not sure if is enough to call it a special location. I don't know so well the shape of astrocytes, but it seems express in the cytosol. Also, it is important that you review the pattern expression of your antibody, sometimes the manufacturer specifies the location where the antibody labels,
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