I am currently conducting experiment on determination of total flavonoid content by using Quercetin as standard. After reading few literature, I found out some researchers used 415 nm and some used 510 nm as maximum wavelength. My question is which wavelength should I follow and why? I noticed different wavelength used for different methods even though it was for same standard in this case Quercetin. For example, AlCl3-NaNo2-NaOH method used 510 nm while AlCl3-Methanol method used 415 nm. Someone please clarify this. Thank you.
Are you dissolving quercetin in a methanol solution?
There are lots of investigation stating that the best transition energy related to π -> π * for quercetin is around 370-380 nm.
However, on the basis of the protocol you are following (different reagents, buffers and chemicals) you could obtain different results.
My best regards,
Gabriele
You can perform a wavelength scan using your reaction mixture and select the wavelength that gives highest absorance/noise ratio.
I think you should perform a wavelength scan and determine the maximum wavelength correlated to your standard and the protocol you are using in your study
Hi Guys, thanks for the advice I tried to do a wacescan but i was not able to even see a little change in absorption in the whole scan, it was the same as my blanco i tried different till very high concentrations of quercetin nothing worked. Who can help me out with this??
I am just making a solution of 95% quercetin solved in 70% MeOH since I use this in all my samples as solvent..
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