Firstly, as concerning the query: HepG2 cell proliferation is boosted by T cells ?, the proliferations of the HepG2 cells, which were separated out from hepato-carcinoma, are lost because cellular cycle demand mechanisms of an organism (read biochemistry of cellular cycle). T cells don't take part in these processes.
Secondly, the methods of MTT assay and MHC-1 Tetramers are used in active immune processes without activity of large doses cytotoxic drugs which suppress immune processes.
Hence, familiarize yourselves with the book entitled „Biophysical and Biochemical mechanisms of organism development in norm and pathology“. This book is published by Publishing House of "Cambridge Scholars Publishing" and is intended for the specialists and the scientists in fields of medicine and biology. This book advances the knowledge of the mechanism development of a lived organism during its lifetime through the normal stationary state and quasi-stationary pathologic state from the viewpoints of biochemistry, biophysics, and thermodynamics. It explores the possibility of estimating experimental results from the point of view giving a broad perspective on the interaction between an organism and its enviroment. The book also describes the biophysical and biochemical mechanisms maintenance stability of internal energy according to the First and Second laws of Thermodynamics in norm and pathology. Especially familiarize yourselves with the Chapters 4, 5, 6 of the book in which there were described mechanisms of oncogenesis, relapse cancer, resistance to cytotoxic drugs by cancer diseases as well as described the offered of the new efficient method cancer treatment creating depression of cancer metabolism and promote activity of the smallest doses cytotoxic substances with exerting activity of immune T cells as comparison with modern methods of chemotherapy with large dosage of cytotoxic drugs. Also the book describes the role of Stem cells in regenerative medicine. Besides the book gives the scientific proof of the immortality of a human soul after death.
Also attached in email the announcement of book can be viewed with commentaries via website: https://www.cambridgescholars.com/product/978-1-5275-0711
Immunotherapy - mode of action
Teaches the immune system to identify and destroy cancer cells. Immunotherapy can be given as a medication or infusion.
Chemotherapy - mode of action
Kills rapidly dividing cells, including cancer cells and healthy cells. Chemotherapy is administered in cycles.
Side effects
Immunotherapy
Side effects can include nausea, fatigue, inflammation, and skin rash. These side effects are often less predictable than chemotherapy side effects.
Chemotherapy
Side effects can include nausea, hair loss, mouth sores, and fatigue. Chemotherapy can also damage healthy cells, such as those in the skin, blood, and intestines.
Response time
Immunotherapy: May take longer to have an effect because the immune system needs time to ramp up.
Chemotherapy: May provide quicker results because it directly attacks cancer cells.
Length of treatment
Immunotherapy: Can work for an extended period because the immune system remembers what cancer cells to look out for.
Chemotherapy: Works for as long as the cancer drugs are in the patient's body.
A tumor arises under the following conditions: The immune system is immunomodulated by an external cause and does not recognize defective cells and does not mark them for elimination. I myself have observed this change not only in myself, but also in other patients after a wild viral disease. If the tumor grows to a larger size of 1 to 6 cm, the organism is not able to clean up the defective cells. I am convinced that it is necessary to surgically remove as much of the tumor as possible. The cells that remained after resection must be killed by chemotherapy with the greatest possible load, taking into account the state of health. After chemotherapy repeated 3 times, it worked for me to leave a break of 6 weeks for the organism to recover. To prevent cancer from recurring, block 1/4 of the recycling of B-lymphocyte programming in the lymph nodes. The lifespan of blood cells is 120 days. 1/4 is a month. If incorrectly modulated B-lymphocytes die before they can pass on the defective information in the lymph nodes to naive B-lymphocytes. Experimentally, according to the calculation, 4 times 1/4 die out and innate immunity returns. We have many drugs that can interrupt immunity. The article is only partially verified so far. There are still 2 years left to verify whether metastases will return. If I do not live, it was my wrong judgment and the wrong technological treatment procedure. I will inform you further, the result of PET CT will be especially important.
Thank you for reading Jan Jeník
I don't agree with your opinion that the immune system is immunomodulated by an external cause and does not recognize defective cells and does not mark them for elimination. Firstly, these your opinion is contradicts to modern scientific tendency to use immunotherapy treatment cancer. Secondly, the internal works of cleaning organism from dead cells via autophagia is made by immune system too. Please, read in my published works, some mechanism of resistance cytotoxic drugs after modern method treatment with large dosage cytotoxic drugs of cancer disease: This my book can be viewed with commentaries via website: https://www.cambridgescholars.com/product/978-1-5275-0711-1
Thank you for your response. Science is always about new knowledge and denies the original knowledge. According to current knowledge and possible treatments, I was supposed to die in 3 to 6 months. Remotely within the framework of lifelong medical education, I risked my new view. I do not disparage existing knowledge in any way. Current studies on DNA are like a bushy tree and the trunk cannot be seen through the leaves. Only a new opinion, although I agree that it is contradictory, I risked trying it. Status: Lung tumor grown into the aorta, size 5 x 6 cm, type NSCL (although hopeless). At my own request, operated on, resected margins 55% PD-L1, metastases present, spread through the lymph nodes. Proven cause: Wild Covid 19 disease associated with hospitalization. X-rays compared before the COV 19 disease, clean lung lobes. With Cov 19, no signs of tumor. Recovery followed, accompanied by cough and exhaustion. Still monitored at a specialized Faculty department. At the next check-up in 3 months, a tumor had formed. Apparently from a localized focus of PostCovid inflammation. Before I was placed in a specialized oncology department, the tumor had become incurable. Here, there is no discussion of the influence of immune system failure. It is important to remember that the first historically successful treatment in 1896 was through the induction of the immune system.
Successful treatment was practiced by doctor Colle (inaccuracy of the name possible) by injecting weakened streptococcus. He induced a febrile illness and completely cured many patients. In order not to lead others to the wrong conclusion, I am waiting for a total of 5 years to live. So far, this is an insufficiently substantiated opinion. Status 3 years without tumor and return of metastases. So tumor = 0, metastases = 0. I do not claim that this is a cure-all treatment, but it is my duty to point it out. If I die within two years, it was a wrong judgment. I have read your work with thousands of others and I definitely consider it highly beneficial. My medical invention is being monitored by experts from the university hospital, oncology department. But the procedure was not kept secret and several hopeless patients followed me. Their tumors stopped growing and metastases are starting to decrease. I would like to point out that as a scientist who was more than 10 times the best in the world (outside the field of medicine), I am obliged to inform. The results of my inventions have also been successfully used in cosmic bodies.
I am unknown because most of my scientific work is bound by confidentiality according to contracts with the client.
However, this does not mean anything, I could be wrong like many other people.
Thank you for your response and I welcome a different opinion on the matter.
What matters is not what I think, but what I measure.
With friendly greetings!
Jan Jeník
I don't deny modern chemotherapy of using large dosage cytotoxic drugs for cancer treatment. However there are deficiencies of using only modern chemotherapy in forming cancer metastases in neglected state of an organism or appearance after chemotherapy with large dosage cytotoxic drugs forming either resistance cytotoxic drugs or relapse cancer disease. As opposed to modern chemotherapy, I propose new method cancer treatment which eliminate all these deficiencies. Now I prepare paper entitled "The benefit of Clinical Trial for the new method cancer treatment as opposed for modern chemotherapy of Cancer Therapy" with supplemental explanation these arguments for published data in my book entitled "Biophysical and Biochemical Mechanisms of Organism Development in Norm and Pathology" (see website above).
The only immune therapy of cancer disease cannot substitute for replace complete chemotherapy of cytotoxic drugs because power of immunotherapy is deficient to stop development of creating cancer metastasis. However the large dosage cytotoxic drug suppress activity of immune system. Therefore the new method cancer treatment use very small dosage cytotoxic substances in state of combination “Prolonged medical starvation 42 - 45 days of the organism” with considerably decreased dosage of cytotoxic drugs which gives possibility to use also activity of immune and hormonal systems. Please, read the my book through website: https://www.cambridgescholars.com/product/978-1-5275-0711-1
Your book is not available in electronic form. Neither in my favorite library: Princeton University LIBRARY nor in professional publishing websites. NIH National Library of Medicine PubMed central.
The book is not a contribution to me. If you have an editorial template in *.doc or *.PDF I will be happy to send you those few dollars.
If you have no fund to buy the book, you can receive this book either in City Central science Library or in University Library, It can not be that these Library did not purchase the books published by Publishing House of "Cambridge Scholars Publishing" and is intended for the specialists and the scientists in fields of medicine and biology. I think that you will interest yourself obtaining this book.
Hello. Thank you for the information. I get paid well for inventions from all over the world. The problem is that in our country people borrow and read books in electronic form. Your printed book is not registered in any Czech specialist library. I have arranged another professional workplace by purchasing a house in the mountains (a former mountain hotel on the smuggling trail to Poland). It is beautiful here and sometimes deer look into my laboratory through the window. Wonderful peace for studying and working. I like to ski to the ski resort, it is 20 minutes away and I like to ride a mountain bike in the mountains. It is a 6-hour drive to the city library by car. Of course, I also have a business apartment in the headquarters of a privately owned company in Prague-West, where I commute to check on employees. But I have a lot of work and little time. The Czech telecommunications operator has set up a long-distance wireless high-speed Internet connection for me in the mountains. The connection is enough for eight computers in operation.
I appreciate your work and all those who are trying to further advance science. Unfortunately, the seller GB is not in the European Union, has VAT: GB108280727 and since I am registered under VAT CZ5408082724 it means going to customs in person and paying customs fees, that's a lot of paperwork and a lot of time. If you don't violate the publisher's terms and conditions, send me a brief annotation, that will be enough for me. Thank you, best regards Jan Jeník
Well, you can buy the book in paperback for USD 39,99, instead normal hard book for USD 78,99, making order in Google through website: https://www.cambridgescholars.com/product/978-1-5275-0711-1
I checked your VAT account.
Result:
Although Cambridge Scholars does not charge its authors, it has been listed as a predatory publisher on Beall's List and on Cabells' Predatory Reports
https://web.archive.org/web/20230918100210/https://predatoryreports.org/news/f/is-cambridge-scholars-publishing-predatory
The fact that it was not possible to purchase a scientific work electronically made me suspicious.
Best regards.
No, cytotoxic chemotherapy with large dosage cytotoxic drug damage both cancer cells and immune cells with also hormonal system and even complete immune system. Please, remember the state of sick man after complete Chemothrapy with large dosage cytotoxic drug.
Energy and entropy don't based on unknown molecular constitution and thermodynamic can understand only through know of three thermodynamic laws. Please, read my book through web-site: https://www.cambridgescholars.com/product/978-1-5275-0711-1
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