How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

NVT or NPT is preferable for gas adsorption simulation ?

Hi there, In order to perform molecule dynamic simulation on gas adsorption on substrate materials, NVT or NPT ensemble is preferable ? Thanks!

08 February 2021 173 1 View

Option "cutoff-scheme" not recognized by Gromacs 4.6.5?

Hi, I got the following error message while running parallelized simulations with OpenMP: OpenMP threads have been requested with cut-off scheme Group, but these are only supported with cut-off...

02 January 2021 4,564 5 View

Can I use gelatin to pre-coat the plate for HepG2?

I am working with HepG2 cells, and I want to pre-coat my flask/plate to keep the cells in single layer. Can I use gelatin to coat the dish or I need to use collagen? I was wondering if any of you...

15 December 2020 5,599 3 View

Did anyone do microglia Oil Red O staining?

I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...

24 November 2020 2,359 2 View

How to calculate UTCI using ERA5 dataset?

17 November 2020 2,127 3 View

Could anyone professional help me to solve this problem?

I have installed the software BRUKER Flexanalysis 3.4 on my computer successfully. But when I tried to import the MS data produced by BRUKER MALDI-TOF-MS, I met with an error described in the...

10 November 2020 8,205 1 View

Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Does good inferential comprehension first require solid literal comprehension?

Hello, This can be a silly question to ask, but my main field is not in reading comprehension. While I try my best to phrase my question, it can sound a bit awkward. Thank you for your...

11 November 2020 811 3 View

How are the 3 levels of reading comprehension reflected in grade-level English Language Arts curriculum?

Hello, How are the 3 levels of reading comprehension (literal, inferential, and critical) reflected in grade-level English Language Arts (ELA) curriculum? In particular, normally, is literal...

01 November 2020 1,240 4 View

Is the level of cognitive flexibility positively correlated to the level of reading comprehension?

Hello, Is the level of cognitive flexibility positively correlated to the level of reading comprehension (greater cognitive flexibility brings higher level of reading comprehension)? If there is a...

01 November 2020 8,254 5 View

Is there a reading comprehension theory that suggests simple reading questions are best solved via simple text analysis, and vice versa?

My main field is not Linguistics/reading comprehension so what I am asking might sound a bit silly or not make a lot of sense. My question could certainly be better-phrased. Is there a theory in...

23 October 2020 8,310 2 View

First time project advice: How to close transcription factors, SOX9 and NFIB, into baculoviral vector, pEZT-BM?

Hi all, I am new here and I am about six weeks in to my first ever research project! (I just came here to kick-start my career as a researcher.) :) The aim of my research project: "The aim...

15 September 2020 9,890 3 View

Could you collaborate, please?

Hello everyone! I've developed a 35-item questionnaire to measure ESP students' critical reading strategies awareness while reading materials in English (as part of my Ph.D. research). Now, I need...

27 June 2020 2,484 3 View

How do you read the papers without falling into a rabbit hole?

Often while reading a paper, I will see a citation that that seems more relevant than my current one. I'll switch to the new paper, only to experience a similar feeling. Or maybe I need to look...

11 May 2020 3,883 23 View

How to compute PISA 2018 tests scales?

Good Evening, The results of the PISA 2018 test were published at the end of 2019 on the OECD website (https://www.oecd.org/pisa/data/2018database/). Among the available data are the Code book...

26 February 2020 7,507 3 View

Is it worth to study the effect of item format on psychometric characteristics of the items?

In my work I would like to study the effect of item format on psychometric characteristics of the items in reading comprehension assesment. At the moment, feedback concerning the theme of my...

15 December 2019 9,249 3 View