I did a dpph method and make a variety of concentration (400, 200, 100, 50, 25 ppm). I have done it by making 1000 ppm first and diluted them, I already did this 4 times but the results are always same. is there something wrong with my way in diluting the sample? or is there something wrong with my sample?
Your sample may not contain active compounds capable of scavenging the DPPH radical.
Rafina Naqiyya Qatrunnada Different concentrations ranging from 400 to 25 ppm can hardly have same DPPH radical scavenging potential. The concentration of metabolites will definitely be decreasing.
Have you checked the absorbance value of the 1000ppm stock solution you are preparing initially? If not do check the absorbance and see.
Also, how are you diluting the sample solution into different concentration?
consider another way to create the concentration gradient Rafina Naqiyya Qatrunnada
The stard of high concentration depends on the antioxidants content is it high or not, i prefer start at 1000ppm
Might be its due to solubility, check the solubility of product.
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