Tetrazolium/Formazan test method?

More Desalegn Amenu's questions See All

Is that any simple techniques to determine milk protein without kjeldahl method.????

Hello, Scintissist. I'm looking for a quick and easy way to detect protein or nirogen levels in milk without using the Kjeldahl method, so do let me know if you know of any.

11 May 2023 592 2 View

Does MALDI TOF detect and identify bacteriocin types from Cell free Supernatant?

Hello there, dear scholars. Allow me to present my challenges. Is MALDI-TOF capable of detecting and identifying bacteriocins such as nisin and pediocin? THANK YOU SO MUCH!!!

27 March 2023 7,901 1 View

MRS inhibitor for fungi?

Does the benzoic acid used as antifungal agents on to MRS agar?

18 March 2023 3,188 1 View

Discourse analysis and content analysis, how do I use both together to analyse data from two sources?

Currently, I am writing my article based on data extracts from teachers' interviews and video recorded classroom interactions. I developed themes and categories of both data inductively. I am...

13 March 2023 3,831 16 View

What is the impact of culture in nation building?

Cultural management is believed to be one of the main factors that determines the success of nation building.

05 March 2023 5,941 4 View

Bacterial species identification methods?

Hello Dear scientists, Please would you suggest me an idea on bacterial species identification to genus level, hence due to some financial shortage, i couldn't proceed to bacterial sequencing...

07 January 2023 3,795 3 View

What is an acceptable ratio of treated-to-control sample size in a Propensity Score Matching based impact evaluation?

It is intuitive to me that in a 1:1 proportion of treatment: control groups sample size would be problematic if a treatment observation fails to have a corresponding match from the control...

19 November 2021 5,524 2 View

What is the best transport media for anaerobic bacteria (lactic acid bacterai)?

I need to transport lactic acid bacteria using anaerobic transport media like carl Blair and Thioglycolate broth , so rather than this media or other else what is the best appropriate...

17 October 2021 6,605 3 View

Any formula or method to calculate yield loss caused by each plant diseases when more than one diseases occurred simultaneously on a plot/genotype?

In plant pathology experiments, some times multiple diseases can occur on a single plot even on a single plant at the same time and growth stage of the crop. Finally it becomes difficult to...

21 September 2021 4,026 4 View

What is a free market or market economy?

Market economy is one of the economic models which is mainly applied in the West. There are definitely differences among the market economy model and the other economic models. It is thus...

02 January 2021 7,577 27 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,439 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,752 3 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,937 1 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

I want to plot calibration curve of pure mirtazapine powder using HPLC. Can someone please suggest a method without HPLC buffer as a mobile phase?

I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...

14 July 2024 1,493 3 View

What binders suggestion that I can use for zinc alloy?

I am working on powder metallurgy, and during the compaction process, the powder does not pelletize and remains in its powder form. To address this issue, I am considering using binders to improve...

13 July 2024 9,003 2 View

How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

10 July 2024 2,462 1 View

Can anyone suggest what amount of powder I have to disperse in the solvent for taking the FTIR spectra?

I have to compare the FTIR of the two samples, one is in powder form and other one is the powder dispersed in IPA or water. Both are same material, so how the bond will change

09 July 2024 8,011 2 View

Is VSM measurment of NdFeB alloy powder sample require any spesific sample preparation ?

Is any specific sample preparation required to perform VSM measurement of NdFeB alloy powder to get a square-shaped M-H loop? Or is it mandatory to make magnetically aligned and sintered pellets...

09 July 2024 1,967 0 View

Giada Mele

Hello, I think you can use MTT powder to evaluate cellular metabolic activity after treatment.

Michał Karol Pierański

During our laboratory course for students we use paper discs soaked with TTC. You can try them.

Khem Raj Meena

MTT is the best. U can try. We use for mammalian cell lines.

Same may be used for antimicrobial activity on the basis of color produced by cells and thereafter take reading in ELISA