Hello Dear Scientists, I need to use Tetrazolium/Formazan test method to evaluate antimicrobial activity of Bacteriocin, but I cant get TTC powder, so what is the possible reagents or salt could be used in place of TTC?
Thanks a lot
Hello, I think you can use MTT powder to evaluate cellular metabolic activity after treatment.
During our laboratory course for students we use paper discs soaked with TTC. You can try them.
MTT is the best. U can try. We use for mammalian cell lines.
Same may be used for antimicrobial activity on the basis of color produced by cells and thereafter take reading in ELISA
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