How to provide reliability in P2P storage system?

I am familiar with a fact that in P2P storage distributed systems reliability is provided with redundancy using erasure code or simple file replication. I am interested if there are some other...

05 February 2021 1,325 3 View

How do forests influence avalanches and how protecting of the avalanches?

How do forests influence avalanches and how protecting of the avalanches? Forests play a very important protective role related to the avalanches. They act as a safeguard for communities and...

26 December 2020 9,167 1 View

How to track users accessing desktop file system?

I want to log user activity in a way to determine how they access their files on daily basis. For example, student have book in pdf and he read this file and other files during learning process....

04 October 2019 942 2 View

How to calculate the item characteristic curve for a polytomous IRT model?

Most software packages for analyzing polytomous models under the Item Response Theory approach show option characteristic curves as an output. Given that I have the data on those option...

14 October 2017 8,379 10 View

Can restriction enzyme integrate cytosol when transfecting cells with lipofection for establishing stable cell lines?

10 August 2016 6,920 4 View

Can restriction enzyme integrate cytosol when transfecting cells with lipofection for establishing stable cell lines?

10 August 2016 9,920 2 View

How do I apply multiple imputation on incomplete datasets?

I'm working on a dataset of variables and I would like to apply a multiple imputation on a subset of variables of great interest. I'm working on STATA. I also know what's the prinicipal of the MI...

31 May 2015 3,276 4 View

Which serum free media can I use for Cos-1 cells?

I would like to do cell uptake experiments using Cos-1 cells in serum free conditions. Which media can I use?

24 May 2015 7,347 1 View

Small molecule dissolved in 100% DMSO formed crystals in culture media. Can I use beta-cyclodextrin derivative as a solvent for Vitro study?

14 July 2014 3,065 3 View

No title

I am doing a time-dose-mortality test on an insect with neem oil. For analysis, I would like to use the Cox regression model from IBM SPSS 20 for Lethal Dose 50 (LD50) determination. I would like...

11 June 2014 10,004 2 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Can anybody suggest safest irreversible inhibitor for cholinesterase (EC:3.1.1.7)?

I am working on the assay of brain acetylcholine. I need an irreversible inhibitor of cholinesterase that is relatively safer (not volatile, preferably solid) to use. This is to prevent the...

08 December 2020 3,797 3 View

Do you know anyone who might be interested in running oncology related randomized studies concerning vit. D as profilaxex for oncological problems??

There have been a number of studies relating vitamin D deficiency to cancer and relapse in different types of cancers, for instance Hodgkin's lymphoma. Yet it does not have any repercussion for...

09 November 2020 9,211 2 View

Anyone interest to apply, please go on?

04 October 2020 1,239 2 View

What is the best way to keep tissue samples in during dissection for ELISA (besides snapfreezing in LN2)?

Hi, I wonder if there is any buffer that i can keep tissues in, right after the dissection (mice). That would be very handy because there will be a lot of sample collection going on during...

12 August 2020 8,176 3 View

Does using ethanol for de-waxing drosophila heads before dissection interfere with future protein extraction or fly brain imaging?

I have heard that dipping fly heads in 70% ethanol before placing in cold PBS for dissection can help strip the heads of wax and allow them to stay submerged in the PBS during dissection. I was...

10 August 2020 4,373 2 View

Future of cadaveric dissection in anatomy teaching after COVID Pandemic?

Cadaver dissection are the best adopted and accepted teaching tool in anatomy teaching from the accident time till date. Does the dissecting room still have a place in educating our under- and...

02 August 2020 2,948 17 View

Machine learning methods for dynamical systems?

I am comparing different machine learning techniques for learning dynamical systems (e.g. a system of ordinary differential equations), and so far I've used Long-Short-Term Memory Networks (LSTM)...

29 June 2020 3,633 5 View