Hi,

I'm familiar with using Trypsin-LE to disassociate adherent cells from tissue culture (TC) plastic, but I'm interested in using Mag-Trypsin (Immobilized TPCK-trypsin magnetic beads - (takarabio.com) to disassociate cells from TC plastic too. However, this trypsin product is used for protein digestion for mass spectrometry, and not typically for cell culture protocols. Has anyone used Mag-Trypsin for cell culture and got it to work successfully?

I recently added 100 ul of Mag-Trypsin to a 24-well plated with 100K HEK293 cells and incubated for 10 mins (37oC, 5% CO2, 8% O2). After the incubation and after separating the beads using a magnet separator, I replated the separated liquid (which I believe is storage buffer containing cells?) with 900 ul of DMEM+10% FBS growth media into a new, fresh TC well. After 24 hours, there are HEK293 cells in the replated well, but they're not adhering, and I see a few magnetic beads too that did not get separated using the magnet. Wondering if anyone has any insights or protocols to help me get cells to properly replate onto TC wells and look healthy after using Mag-Trypsin?

I appreciate it! Thanks