Dear Eric,

If you have phage, for example, in SM buffer (100 mM NaCl, 25 mM Tris-HCl pH 7.5, 8 mM MgSO4, 0.01% (w/v) gelatin), filtered through 0,22um filter, then it's a "mixture" of proteins and DNA, so you can make the simpliest phenol extraction. This method has been used a lot of times in my case, always fine.

Good luck!

Regards,

Evgeny.

Thank you, Evgeny!

Hi, I tried the method mentioned by Dr. Klimuk, after enzyme digestion, the DNA been extracted seems to be BL21 bacterial genomic DNA, which can not be cut in appropriate sizes. T7 phage needs to propagate in BL21 bacterial cells, so I was wondering how to separate the T7 phage genomic DNA and bacterial genomic DNA? Thanks a lot.

Dear Eric,

In my laboratory, we earlier used a method similar to phenol extraction proposed by Dr. Klimuk, previously modified by digesting E. coli DNA by DNAse and thermal denaturation of the phage proteins. This may solve the host DNA problems observed by Jiani Xing. At this moment, we use the commercial kit linked above.

https://www.aabiot.com/en/genomic-mini-ax-phage