Hi,

I have recently been a bit confused by the T4 RNA ligases... I know they are being used for 5' and 3' RACE, and also for RNA end labeling... Does anyone know if I can also use them to tag total RNA at the 5' or 3' end with some RNA oligos, perhaps about 20mers or so? would I have to do some modification on the RNA, like removing phosphate caps?

Thanks!

Basak