Basak Ozturk

5 Questions 19 Answers 0 Followers

Questions related from Basak Ozturk

ssDNA electrophoresis

Hi, Does anyone have an idea how to run ssDNA on agarose gels? I tried my regular protocol, but I am suspecting that the ssDNA is forming some secondary structures..

20 March 2009 8,034 1 View

PCR with single primer

Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...

12 March 2009 7,246 6 View

assays and data interpretation

Hi, there was recently a question about real time results and how to interpret them. I would recommend the paper: Quantification of mRNA using real-time RT-PCR, from Nolan et al., Nature...

06 February 2009 9,360 0 View

T4 RNA Ligases

Hi, I have recently been a bit confused by the T4 RNA ligases... I know they are being used for 5' and 3' RACE, and also for RNA end labeling... Does anyone know if I can also use them to tag...

04 February 2009 8,308 1 View

Polyadenylation+RT-PCR

Hi, I have been trying to reverse transcribe total bacterial RNA by polyadenylation and oligod(T) primers... I used the polyA polymerase kit from Epicentre, cleaned up the reaction on spin...

01 December 2008 8,566 4 View