6 Questions 25 Answers 0 Followers
Questions related from Basak Ozturk
Hi, I am trying to transform a bacterium that has not been transformed before.. I am wondering, does anyone have an idea which antibiotic concentration I should take for the selective plates?...
22 October 2009 7,810 12 View
Hi, Does anyone have an idea how to run ssDNA on agarose gels? I tried my regular protocol, but I am suspecting that the ssDNA is forming some secondary structures..
20 March 2009 8,073 1 View
Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...
12 March 2009 7,294 6 View
Hi, there was recently a question about real time results and how to interpret them. I would recommend the paper: Quantification of mRNA using real-time RT-PCR, from Nolan et al., Nature...
06 February 2009 9,409 0 View
Hi, I have recently been a bit confused by the T4 RNA ligases... I know they are being used for 5' and 3' RACE, and also for RNA end labeling... Does anyone know if I can also use them to tag...
04 February 2009 8,364 1 View
Hi, I have been trying to reverse transcribe total bacterial RNA by polyadenylation and oligod(T) primers... I used the polyA polymerase kit from Epicentre, cleaned up the reaction on spin...
01 December 2008 8,616 4 View
