Sybr green PCR product can be used for gel elctrophoresis for confirmation?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Why is my ASO degraded quickly on agarose gel?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Why my colony PCR results of my recombinant bacterial not showing any results?

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Could dyes amplify the spectrum of light to a specific wavelength?

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Robert Adolf Brinzer

Yes you can and you should always run a gel if using a new primer pair to check for off-target amplification.

Cristian Mena

yes you can! obviously useing a the correct gel staining concentration. You can also do a conventional pcr with qPCR primers and just a common Taq ploymerase and the others master mix components.

Halyna Makukh

Yes, you will see in gel with EtBr