Mostafizur Rahman

6 Questions 4 Answers 0 Followers

Questions related from Mostafizur Rahman

Which ct value is Cosider as detected using GoTaq q PCR mastermix (Promega) ?

I had done PCR using GoTaq q PCR mastermix (Promega) for detection Hepatitis B virus cccDNA. For that purpose, which Ct value I will consider as detected or undetected?

04 March 2024 4,966 2 View

How quantitation of cccDNA of Hepatitis B virus can be done by using ct value?

I have done real time PCR of HBV infected biopsy sample by using sybr green master mix using specific primer to detect cccDNA . And also done real time PCR with beta actin gene as a reference...

25 September 2023 8,382 1 View

10 x reaction buffer is same for every PCR reaction?

I want to do rolling circle amplification with phi 29 polymerase enzyme . Previous article show they use 10x reaction buffer. Can I use 10X reaction buffer from thermofisher for the PCR.

15 July 2023 3,575 2 View

I have primer from macrogen which concentration is 100 pmol/ul. How do I convert this concentration into 10 milimole/L?

"primers at a concentration of 10 µmol/L" "primers at a concentration of 10 mmol/L" "primers at a concentration of 0.5 μmol/l" I found this concentration from three different article. How...

12 July 2023 1,519 7 View

Sybr green PCR product can be used for gel elctrophoresis for confirmation?

I want to do Real time PCR using sybr green Master mix. Can I use this PCR product for gel electrophoresis?

14 June 2023 5,184 3 View

Is there any kit available to detect Hepatitis B Virus pre genomic RNA and an Established method to detect Hepatitis B virus cccDNA?

I want corelate Hepatitis B virus PgRNA as an alternative marker. Which has a better predictive value than HBV DNA and well co-relate with intra Hepatic cccDNA.

03 January 2022 7,483 0 View