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Questions related from Mostafizur Rahman
I had done PCR using GoTaq q PCR mastermix (Promega) for detection Hepatitis B virus cccDNA. For that purpose, which Ct value I will consider as detected or undetected?
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I have done real time PCR of HBV infected biopsy sample by using sybr green master mix using specific primer to detect cccDNA . And also done real time PCR with beta actin gene as a reference...
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I want to do rolling circle amplification with phi 29 polymerase enzyme . Previous article show they use 10x reaction buffer. Can I use 10X reaction buffer from thermofisher for the PCR.
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"primers at a concentration of 10 µmol/L" "primers at a concentration of 10 mmol/L" "primers at a concentration of 0.5 μmol/l" I found this concentration from three different article. How...
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I want to do Real time PCR using sybr green Master mix. Can I use this PCR product for gel electrophoresis?
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I want corelate Hepatitis B virus PgRNA as an alternative marker. Which has a better predictive value than HBV DNA and well co-relate with intra Hepatic cccDNA.
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