Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

Does anyone know instruments to measure "mental health literacy" in teachers?

Hi there, I am looking for questionnaires measuring mental health literacy in teachers and educators. does anyone know any studies in this area?

16 September 2020 1,682 4 View

Which solution do you use for low level actinide decontamination of laboratory surfaces like fume cupboards, work tops or floors?

Efficient decontamination of actinides like americium, uranium, neptunium and plutonium from laboratory surfaces. The decontamination should be easy in the sense that it could be performed on a...

03 June 2020 8,707 5 View

What is the best way to organize your MSD V-PLEX Human Proinflammatory Panel 1 raw data for analysis?

Hello People, does anyone have experience with the raw data analyses of the MSD V-PLEX Human Proinflammatory Panel 1? A friend of mine ran 4 plates and struggles with organizing the raw data for...

01 April 2020 3,566 2 View

How to do a non-linear regression analysis with multiple variables and a strong confounding variable?

Hi, I did outgrowth assays on spiral ganglion neurons from the inner ear with each supplemented by different concentrations of two neurotrophins. For each explant I did an image analysis and...

29 March 2020 8,703 7 View

Would somebody have suggestions for bioinformatic tools that predict changes in enzymatic (e.g. kinase) function based on changes in AA sequence?

Prediction of enzymatic activity based on common genetic variation.

19 February 2020 6,582 2 View

What are minimum and maximum values for ratios in Geopolmers (Si:Al, Na2O:H20, NaO:SiO)?

I am trying to perform a screening DoE to further study influence of a range of factors for example Metakaolin content on various performances like compression strength and to further optimise my...

23 January 2020 5,020 3 View

How to compare diagnostic efficacy of several methods in a within-subjects design?

Hi, I conducted a study on faking in personality tests, where the participants received two instructions (respond honestly vs. fake good) in a within-subjects design (oder was counter-balanced...

09 January 2020 5,182 3 View

Why do I get a different results (load-displacement-curve) when applying different loads in ABAQUS/Explicit?

I'm currently modelling a steel-concrete composite construction with headed studs in ABAQUS/Explicit and want to analyse the load-displacement behaviour of the headed stud. In the first case I...

18 December 2019 3,070 4 View

What amount of data is necessary to extract meaningful/significant Sequential patterns / rules in a sequential database?

10 March 2019 5,559 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View