What would be best combination of surface coating of Antibody (IgM, IgG, IGD...) for growth of Naive B cell obtained from Mice. what be best concentration we can use to acheive the B cell prolifertaion in 96 well plate.
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
How are the movement of energy in an ecosystem different from chemical cycling and movement of matter through an ecosystem differ from the movement of energy?
03 August 2024 6,134 0 View
Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?
03 August 2024 607 2 View
Why is the cycling of matter important to life on Earth and movement of matter through an ecosystem different than the movement of energy through an ecosystem?
03 August 2024 5,061 3 View
How is matter moved through an ecosystem and movement of energy different from the movement of nutrients through ecosystems?
03 August 2024 4,859 0 View
How the movement of matter is different from the flow of energy and what best describes how matter moves through an ecosystem?
03 August 2024 3,664 2 View
How is energy used differently in chemical and physical changes and difference between the movement of energy and chemicals in ecosystems?
03 August 2024 8,856 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View