Naresh Naik Ramavath

4 Questions 1 Answers 0 Followers

Questions related from Naresh Naik Ramavath

Mice B cell Lymphoma stable cell line generation?

I tried to develop Invitro stable B cell Lymphoma cell line obtained from the mice lymph nodes by postively selecting the B cell (CD45R Beads) and I tried to grow them using IL-4, IL-21, Baff,...

22 February 2025 7,719 1 View

Surface Coating Antibodies for Murine Naive B cell culture?

What would be best combination of surface coating of Antibody (IgM, IgG, IGD...) for growth of Naive B cell obtained from Mice. what be best concentration we can use to acheive the B cell...

08 February 2025 7,926 0 View

How to culture B cell extracted from Lymph nodes/Spleen?

I have extracted B cells from mouse lymph nodes by using selection beads for B cells. I supplemented RPMI medium + IL-4. I found their viability decreasing. Do you have any suggestions for me so I...

12 December 2024 8,920 4 View

How does the Obese diet in NASH/NAFLD mice model can be protective against inflammation?

In my experimental mice model, I have F1 generation from both chow and obese mom. Once I feed them up with obese diet both F1 generation of chow and obese mom. I have noticed lesser inflammation...

20 December 2021 7,961 3 View