How to model the causal effect of working part-time compared to full-time on health?

Hi there, I have to estimate the causal effect of part-time work compared to full-time work on health. I have an unbalanced panel data set with t=2008-2017, except for year 2009. The main issue...

11 December 2020 3,170 3 View

How to correlate bacteria community data with volatile profiles and with fly community data using ordination techniques?

Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...

24 November 2020 5,831 3 View

Does anyone have any advice for Intracellular Staining of methyltransferase for flow cytometry?

Hi All, I have been attempting intracellular staining of a methyltransferase and have been running into issues with consistency. I have attempted sequential staining with a PE secondary and have...

20 November 2020 2,314 1 View

How many casual deaths constitute scientific evidence? Do we know the risks of repeating influenza vaccination?

Italy (2014). Around 35 death after and very closer to the flu vaccination Spain (2020). At least 45 death reported in correlation with adyuvate flu vaccination and 6 time risk to died with...

22 October 2020 2,746 2 View

Use of FcR blocking reagent for B cell surface IgG staining in flow cytometry?

I would like to measure cell surface IgG in human culture of B cells. I have culture of human peripheral blood mononuclear cells (PBMCs) that were immortalized with EBV (Epstein-Barr Virus) and...

30 August 2020 6,805 2 View

After incubating overnight in blocking buffer (BSA) at 4C, how long can i incubate in Primary antibody the following day?

Hello Everyone, I usually block my membrane in BSA blocking buffer for 1 hour before incubating in primary overnight. This time i was pressed for time and decided to block at 4C over night....

05 August 2020 7,007 4 View

Request for suggestion on bimonthly journals in industrial water pollution management ?

Can anyone suggest some bimonthly journals which are either Scopus or UGC indexed in the area industrial water pollution management? thank you?

24 July 2020 2,036 2 View

Which is the most recent phylyogenetic tree for rodents?

Dear all Does someone have a recent rodent phylogenetic tree (preferably in Nexus format or any other format usable in R) or knows a publication with a recent tree? Currently I am still using...

24 May 2020 3,620 4 View

Does anyone know a school offering a BioInformatics short course in Dallas?

I am finding it pretty difficult doing an online program. Does anyone know of any school offering a short program or continuing education in Bioinformatics?

27 February 2020 6,065 3 View

Can I use an antibody with application for FC, to do IP or WB??

Hi there! I need to do immunoprecipitation and Western blot to obtain and detect a protein and I already have conjugated antibodies with FITC and PE for flow cytometry. I was wondering if there...

17 February 2020 223 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View