Can someone please suggest the suitable 96 well filter plate for NHR radiligand binding assays?
I have performed few of the initial experiments for HUVEC cell migration in BD 24 well FluorBlock fibronectin coated plates. I procured the pre-screened primary HUVEC cells from Promocell. I am...
01 February 2015 1,534 8 View
I want to isolate total RNA and protein from the same sample. Isolated proteins should be in native form so that I can use it for further enzyme activity studies. Can someone please suggest any...
05 June 2013 4,481 7 View
I was using Palmitic acid [9,10-3H(N)] from American Radiolabeled Chemical. The end point of the assay is tritiated water which is produced from oxidation of [3-H] palmitic acid. Now they have...
04 May 2013 4,417 0 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View