Dear All, I'm looking for someone who can give me this plasmid: Substrate-free Autobioluminescent Vector (pCMVlux) Applied Stem Cell. Thank you very much
Good morning to all, I have received an invitation to publish a paper from Noves Ricerca Group in a Q2 Journal. They have read my paper and very quickly they have accepted it for publication. It...
20 May 2024 7,407 3 View
como se ve afectado un docente de educación superior por la falta de conocimientos digitales, sus emociones
20 May 2024 1,149 0 View
We recently acquired a ViewSizer3000 from Horiba, and the manufacturer recommended us to use nanopure water from XZero for the NTA analyses. Do you recommend using any other brands of NW? Which...
05 May 2024 1,976 1 View
I've always struggled with concentrating and washing my proteins after purification. Often I lose almost everything in the process. I have used dialysis membranes followed by column filter...
16 April 2024 5,716 0 View
Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...
10 April 2024 9,346 0 View
The old antibody has been discontinued and the currently offered are detecting a band at 62 kDa, which does not reflect the correct molecular weight (47 kDa).
17 March 2024 4,300 2 View
Dear Forum Members, I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing...
10 March 2024 9,081 0 View
What are the current limitations regarding the antimicrobial susceptibility methods, standard procedures implementation, and workplace communication between the clinicians and the microbiology...
06 February 2024 6,318 4 View
The buffer contains: 100mM NaCI, 0.1% Triton X-100, 300mM Sucrose, 1mM MgC2, 1mM EGTA, 10mM PIPES (pH 6.8), and 100uM PMSF
23 January 2024 4,464 1 View
In public life, democratic mechanisms can be misused. Fitting electoral rules to desired results is called Gerrymandering to remember a prominent politician who was an expertin such Machiavellian...
03 January 2024 2,849 0 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View