Strain sweep Rheology problem?

More Deepan Chakravarthy's questions See All

Problem in Williamson Ether Synthesis?

I am trying to prepare ether from 2-Phenylethyl iodide with benzyl alcohol by Williamson Ether Synthesis. I tried NaH method, K2CO3 method for the preparation. But all cases I end up with major...

10 November 2018 8,770 3 View

How to prepare N-LipoylLysine ?

I tried to prepare N-LipoylLysine by solid phase as well as solution phase. Biggest challenge is ring opening polymerization. Most of the time I end up with polymer compounds. Please help me to...

08 September 2018 449 0 View

Need information about RP-HPLC for peptide purification?

Hi Guys, I need a procedure for using reverse phase semi-preparative RP-HPLC. Generally how much time will it takes to purify 100 mg of small peptides. How much solvent is necessary to prepare the...

08 September 2017 2,337 9 View

How to write Z-Matrix for calculating Transition state using multiple basis set for eg 6-31G and lanl2dz ?

Z-matrix

06 July 2013 2,025 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Matthias Knarr

Hi Deepan, one potential option to increase the resolution of your measurement might be to use a different / higher applied frequency. If the frequency is currently to low you have a small signal to noise ratio. Other options might be to use higher concentrations if possible. I hope this helps.

Deepan Chakravarthy

Thank you Dr. Matthias knarr. I will try using higher concentration.