Hello all,

I am collecting muscle tissue samples and would like to extract the nuclear and cytoplasmic protein fractions. The extractions kits suggest using fresh tissue samples as freeze thaw cycles could disrupt the nuclear membrane and contaminate the different fractions. However, we sample >20 animals in 2-3 hours. I would like to process all samples at once or in batches, since individual sample processing may create equipment (centrifuge) availability issues as extraction protocols require 1-2 hours but samples are being collected every ~10 minutes.

I was thinking of collecting the samples and storing them in an "anti-freeze" solution such as glycerol at -20 Celsius until all samples were collected and could be processed all at once or in small batches.

What are your thoughts on this approach? Is there anything else that we can do to resolve our issue?

Thank you!