Hey RG,

I was discussing with a lab member how to sterilize the mineral oil they use for long-term storage of yeast cultures on slants. Currently, they use a home oven to do this via dry heat because of the cloudiness that occurs when an autoclave is used.

My questions are as follows, what is the best and easiest way to do this? Does anything grow in mineral oil in the first place? Are there pros and cons of doing this via filtration or UV?

Thanks!

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