Hi all,
I really would like to know what are the steps in handling stored frozen muscle tissue from liquid nitrogen to dry ice. I am aware of the safety and precautions when dealing with frozen muscle samples from liquid nitrogen and dry ice, but I do not know what to do from the liquid nitrogen phase to the dry ice phase when it comes to muscle tissue.
First of all - what are your plans with the tissue after you have frozen it - i.e do you want to use it for western blotting or for cryosections.
Secondly: How do you freeze your tissue in liquid nitrogen? Do you freeze it as it is or do you put it in tinfoil prior to freezing in liquid nitrogen?
If you are going to use the tissue for western blotting, I would recommend firstly wrapping your tissue in tinfoil prior to freezing and then place it in a plastic bag (bank bag). You can technically place the plastic bag containing your tissue on the dry ice. Nothing should happen.
I agree with Kruger, you need to clarify the purpose for your muscle samples. Technically, it is not a challege to deal with frozen muscle samples from liquid nitrogen to dry ice.
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