Step-by-step procedure for fumigation of a biochemistry lab:
1. Preparation: Gather necessary materials including potassium permanganate (KMNO4), pyridine, formaldehyde, cotton balls, and petri plates.
2. Safety Measures: Ensure proper ventilation in the lab by opening windows and turning on exhaust fans. Wear appropriate personal protective equipment (PPE) such as gloves and a lab coat.
3. Setting Up Petri Plates: Place a small amount of potassium permanganate (KMNO4) in the center of each petri plate.
4. Adding Pyridine: Using a dropper, add pyridine to the cotton balls until they are saturated. Place the pyridine-soaked cotton balls on top of the potassium permanganate in each petri plate.
5. Adding Formaldehyde: With caution, add formaldehyde to each petri plate. The formaldehyde will react with the potassium permanganate and pyridine to release fumes that act as a fumigant.
6. Exiting the Lab: It is crucial to leave the lab immediately after adding formaldehyde to the petri plates. Close the door behind you to contain the fumes within the lab.
7. Ventilation: Allow sufficient time for the fumes to disperse and the fumigation process to take effect. Ensure that the lab is properly ventilated before re-entering.
8. Post-Fumigation Cleanup: Dispose of used materials properly and clean any spills or residues according to laboratory safety protocols.
9. Safety Check: Before resuming lab activities, perform a safety check to ensure that the fumigation process was successful and the environment is safe for work.
Remember to always follow appropriate safety procedures and guidelines when performing fumigation in a laboratory setting.
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