You have to specify what statistical analysis you're referring to. Since you do protein assay, I assume you're just talking about independent t-test

Although independent t-test has underlying assumption of normality in distribution of the dependent variables, it is said that t-test is robust enough to withstand violation of assumption of normality except when the groups sizes differ very much.

If this is the case, you can either transform your data so that ti becomes normally distributed or use a non-parametric equivalent Mann Whitney U test. These are all possible to do using any common stat software like SPSS