What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Hello friends, happy evening !! I am just wondering if you would know where I can find the (ALTS) altruism scale by S.N.Rai and S Singh to download ?

ALTS is an altruism scale developed by S.N Rai and S.Singh.

02 February 2021 7,243 2 View

What does a negative PSNR value between am image and its filtered version indicate?

I understand that because of the log in the equation of PSNR, a negative value is obtained if the value is less than 1. But what does the negative value of PSNR imply?

09 November 2020 392 3 View

What is neutrosophic similarity score (NSS) used for segmentation in image processing? How can it be implemented in Matlab?

Neutrosophic Similarity score is similar to Fuzzy logic. Few papers report using it for image segmentation but implementation details are not provided. If any material regarding this on how it can...

05 November 2020 2,859 3 View

What do you think about Herd Immunity ? Is it Attainable or Unattainable?

Researchers think Vaccine is the only solution .

21 September 2020 8,155 5 View

How to design a circuit for Reconfigurable antenna using PIN diode in hardware?

I have simulated the reconfigurable antenna using PIN diodes using HFSS software.Now for the hardware implementation i am not getting how to give current to the diode. Should i use RLC circuit to...

18 December 2019 6,702 3 View

How can I combine four different items/questions to one variable?

I have four questions and I want to make it one variable. The one variable I wanted to make is work-family conflict. The coding I have 1= several times a week, 2= several times a month, 3= once or...

28 January 2017 9,980 8 View

How to successfully harvest supernatant from EliSpot for further analysis (ie luminex)?

I have limited number of cells and so am interested in harvesting the supernatant from elispot plates to perform further analysis on cytokine profiles. Streeck, Frahm, & Walker (2009) mention...

18 August 2016 192 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View