Standard method for total RNA isolation of bacteria

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What is the significance level for fisher exact test if p value is 0?

in case of chi square testas well if p is 0.00 then how to interpret results and level of significance?

05 May 2024 7,662 3 View

I tried electrospinning poly (2-ethyl oxazoline) with water as a solvent but fiber formation was not promising, should i try other solvents?

voltage- 15 kv, flow rate- 0.5 mL/hr and distance from the needle tip to collector is 12 cm

01 November 2023 5,808 2 View

How the Intercalation of two powder sample can be carried?

If there are two nm size powdered particles, by which process can I disperse both of them into one another so that there properties didn't change?

10 October 2023 8,410 0 View

How can I do FT-IR of zn coated sand with kbr binder?

How will I make the pallet of Zn coated sand with KBr? Means for making pallet we need to crush the sample and KBr in fixed amount properly. So could I do the same thing with sand also? But I am...

30 September 2023 3,278 4 View

I am not able to see drug permeation in MDCK cell permeability experiment, could anybody suggest if I need to modify the protocol?

Hello, Greetings! I am conducting a MDCK cell permeability assay.Below is the protocol I followed : 1. In the apical region, 100 ul cell suspension containing about 33,000 cells were seeded in...

20 July 2023 4,643 2 View

How do I know perfect colour of zno nanoparticle by green synthesised method. After calcination colour were changing ?

I wanted to know perfect colour of zno nanoparticle by green synthesised method. And how many degrees should i maintain in calcination periods below 300 or above 500 degrees.

11 July 2023 5,774 0 View

Please suggest some areas where ML can be applied in Software Engineering?

Topics to initiate...

27 February 2023 6,303 4 View

How to digest sodium which are present in the plant samples?

for practical purposes

19 February 2023 7,127 3 View

How about implementation of ML in Archaeology ? Its challenges....?

Researchers in this domain answer...

17 February 2023 7,503 3 View

How to dissolve PVA in chloroform for binder preparation?

I successfully dissolved 1% PVA in water for 8 hours. Need to know protocol for Chloroform.

22 November 2022 6,438 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View