Hi everyone,

I conducted an in vitro anticoccidial test using an essential oil, following Remmal's protocol. Although the parasite count decreased, the spectrophotometric readings appeared turbid and showed consistent values. I repeated the experiment using an emulsifier; again, the parasite count decreased, the supernatant appeared clear, but the absorbance readings remained identical.

Could this indicate a lack of anticoccidial activity, or is there another factor potentially interfering with the spectrophotometric readings?