Tyrosine aminotransferase expression in HUH7 cells?

05 June 2016 6,388 1 View

Hepatic cell line expressing TAT?

does anyone have first hands-on/practical experience of working woth any hepatic cell line that expresses tyrosine aminotransferase such that activity is measurable. Also, is activity...

04 May 2016 8,738 1 View

What is known about levels of free fatty acids/triglyceride levels in CNS in insulin resistance?

Insulin resistance is known to be associated with higher levels of circulating free fatty acids. Are higher levels of circulating free fatty acids known to be associated with increased levels of...

04 May 2015 9,314 2 View

Another measure of author productivity? Let's call it the X-index.

This is for all those for who the measures of scientific productivity in terms of numbers is important. Can there be another measure which is determined as follows: (Sum of impact factors of...

01 February 2013 1,782 1 View

The Mpemba effect?

Does anyone have explanation for the Mpemba effect?

31 December 2012 1,077 4 View

Glycerol-corrected triglyceride estimates.

I tend to think that for experimental studies, triglyceride estimates should be glycerol-corrected. Would like to know abt your opinion on this.

31 December 2012 2,402 10 View

TRIS for management of hyperglycemia?

This question is just out of curiosity and should not be perceived as mindless promotion of strange ideas for diabetes therapy. Can TRIS, a common base that we all use for preparing buffers be...

31 December 2012 3,205 4 View

Best murine model for induction of insulin resistance by high fat diet

What is the best murine model for the induction of insulin resistance by high fat? C57BL/6J is considered to be a good one. Anyone has any additional insights?

11 December 2012 4,795 9 View

Anti-glucocorticoids for therapy of diabetes?

Do anti-glucocorticoids have realistic potential to be exploited as therapy for diabetes?

11 December 2012 8,121 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View