Hi all,

I would like to use Sortase A (or rather eSRT) to add a fluorescently labelled peptide to my protein.

My plan is:

Produce recombinant target, N-POI-C-LPETG

Where: N = n terminal, POI = protein of interest, C = c terminal, LPETG = sort tag

I will then by some peta glycine peptide from sigma and label the C terminus with cadaverine-Alexa 488

Finally I will use eSRT to attach the penta-glycine-alexa-488 to the c terminus of my POI to give:

N-POI-C-LPET-GGGGG-Alexa488

I am only interested in labeling a small fraction of my sample (0.1 %) so efficiency isn't particularly important.

Does this work plan seem feasible?

Thanks for your input