Sorghum almum seeds?

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Software flowjo and flow cytometry. How to use?

Is there anyone that understands about flowjo software and flow cytometry? I need help to use this software.

02 December 2020 9,996 8 View

Does someone have tips on how to reduce the amount of entrapped oil in the membrane of GUVs with the Phase Transfer Method?

I'm trying to prepare GUVs using the phase-transfer method but apparently I'm getting residual oil entrapped in the bilayer. Did anyone manage to overcome this? Basicaly, I'm following the...

24 November 2020 2,962 1 View

How do you delete a Google Scholar profile?

For some reason, I have two slightly different names associated with my Google Scholar profile. Apparently, both names are associated with the same email. I tried to eliminate one of the names,...

21 October 2020 9,396 3 View

Using facebook for surveys?

Hi, Has anyone had any good (or perhaps bad experience) using a "boosted" survey on Facebook? Has anyone been able to publish using this channel for data collection? Or has anyone been...

12 October 2020 6,697 4 View

Someone has a suggestion to improve the purification of MMLV-RT protein?

I am working with a MMLV-RT (Moloney murine leukemia virus- Transcriptase Reverse). I am expressing in E. coli pTGroE and purifying in a Chelating Sepharose loaded with Ni2+. I am eluting the...

01 June 2020 4,105 4 View

How can you insert measuring items in Projective Techniques?

I am conducting research on consumers' attitudes (namely trust & skepticism) towards Corporate Environmental Responsibility communication and would like to use Projective Techniques (Psychology...

22 March 2020 7,527 3 View

Is it possible to calculate pIC50 of a plant extract?

Is there a way to calculate the pIC50 value of a plant extract? As far as I know, in order to calculate pIC50, the IC50 must need to be in molarity, therefore, it's not possible to get the value...

08 January 2020 8,540 3 View

Why do my protein samples and standards get stuck running Western blot?

When I'm running an 8% SDS-PAGE gel at 100 V, my samples and standards get stuck after a while. Does anyone know why this is happening? Thanks!

20 May 2019 7,797 3 View

Hi, I would like to solubilize a hidrophobic compound in order to administer it to mices. Should anyone suggest a nontoxic vehicle?

The compound is very insoluble in aqueous solution and the compound administration will be given orally. The compound manufacturer has no information regarding the solubility of the compound....

16 January 2019 834 1 View

Anyone can recommend an efficient method for sterilization of corn seeds?

I tried ethanol 70% for 3 min, then bleach 5% for 3 min, followed by 3 rinses of sterile water. Also, tried the same method, but instead of 3 min used 6 min.

10 October 2018 6,640 11 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View