We are going to separate protein, presumably, 10-20 kDa, on nanoLC, and looking for sorbent. We will try first C4, 5 mum, 300 A pore size sorbents. Does it make sense to try C8 phase, 3 mum particle size? What would one expect if use 1000 A pore sorbent? Is it worth using "Reprosil Basic" phase (the proteins are not especially rich in K/R residues)?
Read from the manuals on the topic.
Proteins of this MW range will usually work even on regular C18 material which is used for the analyis of tryptic digests, unless they are very hydrophobic and/or poorly soluble in aqueous/organic. A bigger pore size e,g, 300 A will help. Just make sure to extend your gradient to higher organic percentages and apply suitable amounts - obviously based on your system of detection. If you are looking into a dedicated column, have a look at Dionex monolithic columns - they have shown some very nice separation for small proteins, however these will require a column oven if I recall correctly.
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