Can you tell me if the insert length can influence the PCR reaction? Usually we should use digital PCR (as it is common in real time PCR) for 100-200bp fragments. Is it possible to quantify a plasmid which insert is about 1100bp?
yes, depend on the amount of plasmid number of the copies will give that much band intensity.
Really thank you Tata Santosh!
Also see:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827127/
Really thank you Jack Gallup!!
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