I am running qpcr using Ultra-fast III SYBR Green by Agilent in rocher light cycler 480. My sample is cDNA human cell line. I used reaction volume of 10 ul/20 ul. When I run, no cp value in my triplicate. How is it possible?
Check your internal control or positive control. Then you might rule out some possibilities, and figure out what is going on.
Hello
Could be your template for those triplicate!
Could be mixing problems!
Do you mix your primers, water and mastermix? If not, this will help you to reduce errors.
Do anyone know is it necessary to use low attachement plate instead can I use bacterological petri dish ?
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