Lo Tzu Yu

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Questions related from Lo Tzu Yu

The purpose of combing 1%BSA and 5%non-fat milk for blocking buffer in immunoblotting assay?

The protocol of the antibody from NOVUS　(NBP1-21502) recommends to use the mixture of 1%BSA and 5%non-fat milk as the blocking buffer for Western blot. I am curious the principles and reasons to...

08 August 2018 9,314 6 View

What is the best condition of ferric ammonium citrate(FAC) to hepatocyte (HepG2)?

We would like to treat ferric ammonium citrate (FAC) to HepG2 while there is a problem that the medium or FBS contains ferric/ferrous ion ,either. Should I utilize serum free medium or another...

01 January 2018 2,060 4 View