I have a plant extract (petro.ether).for antibiotic susceptibility test shall i dissolve that in pet.ether or DMSO? which will be good DMSO or pet ether?
petro.ether extract will dissolve well in DMSO. I hope you are using it for in vitro study only, make sure you have a treatment control with DMSO alone.
at https://www.ecoseeds.com/mrsa.html either expressed the plant juice from the fresh plant and put it on a disk, or made a hot-water tea out of dried material, and put that on a disk.