Any Idea please as where to find customized Ab that does not bind to protein A?

I need customized Ab against some antigens and I would like to have isotypes/host variants of the Ab that does not bind to protein A. Is there a company that I could call to discuss customization...

31 December 2016 2,402 4 View

Indexing and DOI for an article?

06 July 2016 4,791 5 View

How could one guarantee in digital PCR that always some partitioned droplets will have no template and will be zero?

Digital PCR relies on proportion of positive and negative results in the droplets. What is the guarantee that not all droplets will have atleast one template and thence all droplets will be...

11 December 2014 3,277 4 View

Any advice on Fluorescent EMSA?

These dCTP fluorescent nucleotides were incorporated into a DNA probe and I ran the free probe in the first lane from the left and cell lysate with the labelled probe in the second lane in 6%...

11 December 2013 4,850 3 View

Loading equal cell extract volume instead of equal amount of protein

I would like to know what is the difference and, the advantages and disadvantages of loading equal volume of cell extract instead of loading an equal amount of protein per lane in SDS-PAGE. I am...

03 April 2013 6,006 10 View

Phenol Extraction of DNA.

I have done phenol extraction and ethanol precipitation of a DNA sample. I have run the DNA on agarose 1% gel and excised the desired band. The band showed good intensity. The excised band was...

02 March 2013 2,718 15 View

Finding the introns as well as the 5' and 3' UTR for a given gene from the cDNA

I would like to find the genomic sequence of a given gene. I have used the NCBI Blast for a sequence from the CDS of the gene and it gave hits corresponding to the location of this sequence on...

31 December 2012 167 9 View

Quantification of protein band density in X-ray films from ECL Western Blots

I have used Image J, Photoshop and Vision Works software to compare protein band densities in x-ray images from ECL-WB, yet there was a huge discrepancy among the results. I think that...

09 October 2012 8,252 18 View

ELISA for a protein secreted by culture cells into growth medium

Does anybody know how to use an ELISA to test for proteins secreted into DMEM growth medium by culture cells? I have the antibodies to these proteins and I would like to know whether I should...

08 September 2012 7,705 35 View

Nonspecific black dots on PVDF after ECL in WB.

Please see attached image of the X-ray film after ECL of the PVDF in WB. The Arrows indicate the black non specific spots in the lower part of the image and the non specific bands which do not...

07 August 2012 5,373 14 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View