Solubilization of eukaryotic cell membranes from pelleted cells

More Mohamed Alalem's questions See All

Any Idea please as where to find customized Ab that does not bind to protein A?

I need customized Ab against some antigens and I would like to have isotypes/host variants of the Ab that does not bind to protein A. Is there a company that I could call to discuss customization...

31 December 2016 2,442 4 View

Indexing and DOI for an article?

How to assign a DOI for an article, please, does anyone have an idea as how to obtain a DOI for my article. Thank you Best regards

06 July 2016 4,838 5 View

How could one guarantee in digital PCR that always some partitioned droplets will have no template and will be zero?

Digital PCR relies on proportion of positive and negative results in the droplets. What is the guarantee that not all droplets will have atleast one template and thence all droplets will be...

11 December 2014 3,317 4 View

Any advice on Fluorescent EMSA?

These dCTP fluorescent nucleotides were incorporated into a DNA probe and I ran the free probe in the first lane from the left and cell lysate with the labelled probe in the second lane in 6%...

11 December 2013 4,898 3 View

Loading equal cell extract volume instead of equal amount of protein

I would like to know what is the difference and, the advantages and disadvantages of loading equal volume of cell extract instead of loading an equal amount of protein per lane in SDS-PAGE. I am...

03 April 2013 6,054 10 View

Phenol Extraction of DNA.

I have done phenol extraction and ethanol precipitation of a DNA sample. I have run the DNA on agarose 1% gel and excised the desired band. The band showed good intensity. The excised band was...

02 March 2013 2,764 15 View

Finding the introns as well as the 5' and 3' UTR for a given gene from the cDNA

I would like to find the genomic sequence of a given gene. I have used the NCBI Blast for a sequence from the CDS of the gene and it gave hits corresponding to the location of this sequence on...

31 December 2012 216 9 View

Quantification of protein band density in X-ray films from ECL Western Blots

I have used Image J, Photoshop and Vision Works software to compare protein band densities in x-ray images from ECL-WB, yet there was a huge discrepancy among the results. I think that...

09 October 2012 8,304 18 View

ELISA for a protein secreted by culture cells into growth medium

Does anybody know how to use an ELISA to test for proteins secreted into DMEM growth medium by culture cells? I have the antibodies to these proteins and I would like to know whether I should...

08 September 2012 7,745 35 View

Nonspecific black dots on PVDF after ECL in WB.

Please see attached image of the X-ray film after ECL of the PVDF in WB. The Arrows indicate the black non specific spots in the lower part of the image and the non specific bands which do not...

07 August 2012 5,421 14 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Do we put respondents data in research methods or findings section of a qualitative study?

Are both ways acceptable, or is there any convention to them? Thanks.

22 July 2024 6,988 4 View

Restaurant research, methods of quantitative and qualitative statistics?

Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...

20 July 2024 2,033 7 View