Hi all,
I want to do Scanning Electron Microscopy on some soft agar plate (0.35% agarose). How can solidify the plate before cutting some pieces and fix them?
I would suggest fixing the sample in 2% buffered gluteraldehye (buffer without NaCl content) , then lyophilise it before further processing for SEM.
This 0.35% agar!! adding 2% of Glut will destroy it. I'm looking for a way to instantlty solidify it before cutting pieces and fixing it with 2% buffered gluteraldehye?? any suggestions?
We have earlier fixed soft gels like alginate-dialdehyde and crosslinked-collagen with 2% buffered gluteraldehyde for SEM processing. Or else, cryoembedding using Leica Jung medium and further freezing could be tried similar to the tissue freezing step in cryosectioning.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Plating