Hello,
for staining the nucleus of living U2OS cells i used SiR-DNA. Before, i synchronized the cells using the double thymidine block. Afterwards the cells were treated with different drugs and then at last stained with SiR-DNA. After 1h SiR-DNA incubation i didn't wash and imaged the cells. The staining of the nucleus was good but i could also see vesicles in the cytoplasm filled with SiR-DNA. Do you know what happened? Were the cells stressed to much, so they habe thrown out the dye from the nucleus? Or did i have an infection with bacteria or something else?
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