Single read vs paired end sequencing in RNAseq?

01 January 2015 0 5K Report

Which is the advantage of performing a paired end sequencing instead of a single read one?

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Science method

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

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Can you connect an HPLC to a Mass Spec only at a certain time point?

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Separation of organic acids-HPLC?

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How can we use mobile apps for improving students' academic performance?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

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How to improve peaks separation in HPLC?

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How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

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Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

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AGILENT HPLC 1100 SERIES , any expert available please?

AGILENT HPLC 1100 SERIES operation assistance needed.

25 July 2024 10,045 2 View